Plasmid

Part:BBa_I713026:Design

Designed by: Zhenyu Shi   Group: iGEM07_Tsinghua   (2007-10-26)


pEB-Lambda Amp-


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 381
    Illegal XbaI site found at 1185
    Illegal XbaI site found at 1290
    Illegal SpeI site found at 258
    Illegal PstI site found at 403
    Illegal PstI site found at 1250
    Illegal PstI site found at 2440
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 740
    Illegal SpeI site found at 258
    Illegal PstI site found at 403
    Illegal PstI site found at 1250
    Illegal PstI site found at 2440
    Illegal NotI site found at 1271
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2227
    Illegal BamHI site found at 252
    Illegal BamHI site found at 415
    Illegal XhoI site found at 1278
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 381
    Illegal XbaI site found at 1185
    Illegal XbaI site found at 1290
    Illegal SpeI site found at 258
    Illegal PstI site found at 403
    Illegal PstI site found at 1250
    Illegal PstI site found at 2440
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 381
    Illegal XbaI site found at 1185
    Illegal XbaI site found at 1290
    Illegal SpeI site found at 258
    Illegal PstI site found at 403
    Illegal PstI site found at 1250
    Illegal PstI site found at 2440
    Illegal NgoMIV site found at 1616
    Illegal NgoMIV site found at 2891
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 851
    Illegal SapI.rc site found at 2740
    Illegal SapI.rc site found at 2950


Design Notes

delete a part of the Amp(r) gene from BBa_I713025


Source

BBa_I713025

References